Integrative study of subcellular distribution, chemical forms, and physiological responses for understanding manganese tolerance in the herb Macleaya cordata (papaveraceae).

Macleaya cordata is a perennial herb, a candidate phytoremediation plant with high biomass and manganese (Mn) tolerance. To study the mechanism underlying its Mn adaptability, Mn2+ at various concentrations (0, 1000, 5000, 10000, 15000, and 20000 µM) were applied to M. cordata to investigate the subcellular distribution and chemical forms of Mn, as well as the resulting physiological and biochemical changes by pot culture experiment under natural light in a greenhouse. According to our results, Mn level in M. cordata increased with exogenous Mn concentrations; and Mn contents in different tissues exhibited a leaf > root > stem pattern. Meanwhile, biomass and the level of photosynthetic pigments increased at lower Mn concentrations but declined as Mn concentration further ascended. Subcellular distribution analysis revealed that Mn was sequestered in cell wall and vacuole; in addition, it was incorporated into pectates and protein, phosphates, and oxalates. These findings revealed a possible effective strategy for M. cordata to reduce Mn mobility and toxicity. Moreover, a continuous boost in the level of malondialdehyde was observed with Mn gradient; whereas contents of soluble proteins and proline, and the activities of superoxide dismutase and peroxidase were initially increased and then dropped. Altogether, these results indicated that most Mn was trapped in the cell wall and soluble fractions in low toxicity forms such as pectates and protein, phosphates, and oxalates. These strategies, that is functioning cooperatively with the well-coordinated antioxidant defense systems and the non-enzymatic metabolites, confer strong resistance to Mn in M. cordata.

Corn poppy (Papaver rhoeas) cross-resistance to ALS-inhibiting herbicides.

BACKGROUND: Papaver rhoeas (L.) has evolved resistance to tribenuron in winter wheat fields in northern Greece owing to multiple Pro(197) substitutions. Therefore, the cross-resistance pattern to other sulfonylurea and non-sulfonylurea ALS-inhibiting herbicides of the tribenuron resistant (R) and susceptible (S) corn poppy populations was studied by using whole-plant trials and in vitro ALS catalytic activity assays. RESULTS: The whole-plant trials revealed that tribenuron R populations were also cross-resistant to sulfonylureas mesosulfuron + iodosulfuron, chlorsulfuron and triasulfuron. The whole-plant resistance factors (RFs) calculated for pyrithiobac, imazamox and florasulam ranged from 12.4 to > 88, from 1.5 to 28.3 and from 5.6 to 25.4, respectively, and were lower than the respective tribenuron RF values (137 to > 2400). The ALS activity assay showed higher resistance of the ALS enzyme to sulfonylurea herbicides (tribenuron > chlorsulfuron) and lower resistance to non-sulfonylurea ALS-inhibiting herbicides (pyrithiobac > florasulam ≈ imazamox). CONCLUSION: These findings indicate that Pro(197) substitution by Ala, Ser, Arg or Thr in corn poppy results in a less sensitive ALS enzyme to sulfonylurea herbicides than to other ALS-inhibiting herbicides. The continued use of sulfonylurea herbicides led to cross-resistance to all ALS-inhibiting herbicides, making their use impossible in corn poppy resistance management programmes.

Self-incompatibility in plants.

Sexual reproduction in many flowering plants involves self-incompatibility (SI), which is one of the most important systems to prevent inbreeding. In many species, the self-/nonself-recognition of SI is controlled by a single polymorphic locus, the S-locus. Molecular dissection of the S-locus revealed that SI represents not one system, but a collection of divergent mechanisms. Here, we discuss recent advances in the understanding of three distinct SI mechanisms, each controlled by two separate determinant genes at the S-locus. In the Brassicaceae, the determinant genes encode a pollen ligand and its stigmatic receptor kinase; their interaction induces incompatible signaling(s) within the stigma papilla cells. In the Solanaceae-type SI, the determinants are a ribonuclease and an F-box protein, suggesting the involvement of RNA and protein degradation in the system. In the Papaveraceae, the only identified female determinant induces a Ca2+-dependent signaling network that ultimately results in the death of incompatible pollen.

Elicitor-activated phospholipase A(2) generates lysophosphatidylcholines that mobilize the vacuolar H(+) pool for pH signaling via the activation of Na(+)-dependent proton fluxes.

The elicitation of phytoalexin biosynthesis in cultured cells of California poppy involves a shift of cytoplasmic pH via the transient efflux of vacuolar protons. Intracellular effectors of vacuolar proton transport were identified by a novel in situ approach based on the selective permeabilization of the plasma membrane for molecules of < or = 10 kD. Subsequent fluorescence imaging of the vacuolar pH correctly reported experimental changes of activity of the tonoplast proton transporters. Lysophosphatidylcholine (LPC) caused a transient increase of the vacuolar pH by increasing the Na(+) sensitivity of a Na(+)-dependent proton efflux that was inhibited by amiloride. In intact cells, yeast elicitor activated phospholipase A(2), as demonstrated by the formation of LPC from fluorescent substrate analogs, and caused a transient increase of endogenous LPC, as determined by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry. It is suggested that LPC generated by phospholipase A(2) at the plasma membrane transduces the elicitor-triggered signal into the activation of a tonoplast H(+)/Na(+) antiporter.